Journal: bioRxiv

Article Title: Synthetic periphyton as a model system to understand species dynamics in complex microbial freshwater communities

doi: 10.1101/2021.10.31.466637

Figure Lengend Snippet: Twenty-six phototrophic microbial species were selected based on their ability to grow under the same conditions and their frequent detection in natural freshwater periphyton. The selected species were then assigned to three groups ( I) . The cell density in the starting inoculum for each group was determined based on the growth rates and reached maximum biomass of each single species ( II) . Groups were added sequentially onto growth chambers covered with the EPS-producing bacteria Sphingomonas elodea . Time points for the addition of the groups and colonization durations for each phototrophic group were determined experimentally (III) (Illustration created with BioRender.com ).

Article Snippet: We therefore used Sphingomonas elodea (ATCC 31461) for surface coating.

Techniques: Bacteria

Journal: Scientific Reports

Article Title: The evolutionary life cycle of the polysaccharide biosynthetic gene cluster based on the Sphingomonadaceae

doi: 10.1038/srep46484

Figure Lengend Snippet: The numbers for the interior branches are bootstrap percentages. The scale bar indicates the number of substitutions per site. The four sphingan-producing strains are S. pituitosa, S. elodea ATCC 31461, Sphingomonas sp. ATCC31, and Sphingomonas sp. WG.

Article Snippet: The genes related to the assembly, polymerization and export and its distribution in 26 genomes, include 22 completed sequenced genomes and four genomes of sphingan producing strains ( Sphingomonas sp. ATCC 31555, Sphingomonas sp. WG, S. elodea ATCC 31461 and Sphingomonas pituitosa ) are shown in .

Techniques:

Journal: Scientific Reports

Article Title: The evolutionary life cycle of the polysaccharide biosynthetic gene cluster based on the Sphingomonadaceae

doi: 10.1038/srep46484

Figure Lengend Snippet: The phylogenetic relationships of S. sanxanigenens NX02 with 25 sequenced strains of the family Sphingomonadaceae (including 21 other sequenced genomes and four sphingan-producing genomes) were constructed using the Neighbor-Joining method based on all core genes. Related genes were predicted by gene annotation and homology alignment against ss and gel genes. “★” indicates the sphingan-producing strains.

Article Snippet: The genes related to the assembly, polymerization and export and its distribution in 26 genomes, include 22 completed sequenced genomes and four genomes of sphingan producing strains ( Sphingomonas sp. ATCC 31555, Sphingomonas sp. WG, S. elodea ATCC 31461 and Sphingomonas pituitosa ) are shown in .

Techniques: Construct

Journal:

Article Title: Structures and Properties of Gellan Polymers Produced by Sphingomonas paucimobilis ATCC 31461 from Lactose Compared with Those Produced from Glucose and from Cheese Whey

doi:

Figure Lengend Snippet: Gellan production (expressed as the concentration of the ethanol precipitate isolated from culture broth), culture OD640, residual carbon source concentration, and broth viscosity (shear rate, 24 s−1) after 48 h of S. paucimobilis ATCC 31461 batch growth at 30°C and 250 rpm in basal S medium containing glucose (○) or lactose (■) at concentrations ranging from 5 to 30 g/liter.

Article Snippet: Strain ATCC 31461 was grown in 500-ml Erlenmeyer flasks containing 250 ml of medium, and the cultures were incubated with orbital agitation (250 rpm) at 30 ± 0.1°C.

Techniques: Concentration Assay, Isolation

Journal:

Article Title: Structures and Properties of Gellan Polymers Produced by Sphingomonas paucimobilis ATCC 31461 from Lactose Compared with Those Produced from Glucose and from Cheese Whey

doi:

Figure Lengend Snippet: Gellan production (expressed as the concentration of the ethanol precipitate isolated from culture broth) (●), residual carbon source concentration (○), broth viscosity (shear rate, 24 s−1) (▴), and OD640 (■) for batch cultures of S. paucimobilis ATCC 31461 grown at 30°C and 250 rpm with 20 g of glucose per liter (A) or 20 g of lactose per liter (B) as the carbon source.

Article Snippet: Strain ATCC 31461 was grown in 500-ml Erlenmeyer flasks containing 250 ml of medium, and the cultures were incubated with orbital agitation (250 rpm) at 30 ± 0.1°C.

Techniques: Concentration Assay, Isolation

Journal:

Article Title: Structures and Properties of Gellan Polymers Produced by Sphingomonas paucimobilis ATCC 31461 from Lactose Compared with Those Produced from Glucose and from Cheese Whey

doi:

Figure Lengend Snippet: Specific activities of PGI, PGM, UGP, UGD, TGP, and TRS in cell extracts prepared from cells of S. paucimobilis ATCC 31461 harvested after 48 h of growth in glucose-containing or lactose-containing media. The bars indicate standard deviations based on at least three enzyme assays for each extract prepared from cells resulting from at least two independent growth experiments.

Article Snippet: Strain ATCC 31461 was grown in 500-ml Erlenmeyer flasks containing 250 ml of medium, and the cultures were incubated with orbital agitation (250 rpm) at 30 ± 0.1°C.

Techniques:

Journal:

Article Title: Structures and Properties of Gellan Polymers Produced by Sphingomonas paucimobilis ATCC 31461 from Lactose Compared with Those Produced from Glucose and from Cheese Whey

doi:

Figure Lengend Snippet: Gellan production (expressed as the concentration of the ethanol precipitate isolated from culture broth) and broth viscosity (shear rate, 0.6 s−1) during S. paucimobilis ATCC 31461 growth at 30°C and 250 rpm in cheese whey diluted 1:4 (○), 1:5 (□), 1:6 (●), 1:8 (■), or 1:10 (▵).

Article Snippet: Strain ATCC 31461 was grown in 500-ml Erlenmeyer flasks containing 250 ml of medium, and the cultures were incubated with orbital agitation (250 rpm) at 30 ± 0.1°C.

Techniques: Concentration Assay, Isolation